7S5K
M. xanthus ferritin-like protein EncB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.490, 44.370, 127.960 |
| Unit cell angles | 90.00, 114.08, 90.00 |
Refinement procedure
| Resolution | 63.440 - 1.950 |
| R-factor | 0.2073 |
| Rwork | 0.205 |
| R-free | 0.25480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5n5e |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.220 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.440 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmeas | 0.200 | 0.950 |
| Number of reflections | 47414 | 4349 |
| <I/σ(I)> | 7.6 | 1.5 |
| Completeness [%] | 98.1 | |
| Redundancy | 6.6 | |
| CC(1/2) | 1.000 | 0.350 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 291.15 | 20% PEG 550 MME, 10% PEG 20000, 100 mM Tris-Bicine pH 8.00, 30 mM calcium chloride |
