7S4Z
Serial Macromolecular Crystallography at ALBA Synchrotron Light Source - Proteinase K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2018-09-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.400, 68.400, 108.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.210 - 1.900 |
| R-factor | 0.16664 |
| Rwork | 0.165 |
| R-free | 0.19559 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ic6 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.513 |
| Data reduction software | CrystFEL (v0.10.0) |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 21010 | 2033 |
| <I/σ(I)> | 17.4 | 0.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3757 | 315 |
| CC(1/2) | 0.999 | 0.226 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 298 | 0.1 M MES pH 6.5, 0.5 sodium citrate, 0.1 M calcium chloride |
