7S4W

Serial Macromolecular Crystallography at ALBA Synchrotron Light Source - Lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	298
Detector technology	PIXEL
Collection date	2018-09-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	79.000, 79.000, 38.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.350 - 2.100
R-factor	0.18359
Rwork	0.178
R-free	0.23225
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vds
RMSD bond length	0.009
RMSD bond angle	1.389
Data reduction software	CrystFEL (v0.10.0)
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.200	2.170
High resolution limit [Å]	2.100	2.100
Number of reflections	7498	716
<I/σ(I)>	9.8	0.5
Completeness [%]	100.0
Redundancy	679	161
CC(1/2)	0.996	0.207

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		298	0.1 M sodium acetate pH 3.0, 18 % (w/v) sodium chloride, 6% PEG 400