7S4A

MRG15 complex with PALB2 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2019-08-30
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 2 21
Unit cell lengths	54.084, 60.157, 131.849
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.671 - 2.693
R-factor	0.2336
Rwork	0.227
R-free	0.29690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2aql
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.750
High resolution limit [Å]	2.693	2.693
Rmerge	0.077	0.650
Rmeas	0.146	0.953
Rpim	0.065	0.434
Number of reflections	12298	603
<I/σ(I)>	10.1	1.7
Completeness [%]	98.7	99.5
Redundancy	4.7	4.6
CC(1/2)	0.995	0.675

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293	Ammonium sulfate, Tris HCl