7S45
Crystal structure of an N-acetyltransferase, C80T mutant, from Helicobacter pullorum in the presence of Acetyl Coenzyme A and dTDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | BRUKER D8 QUEST |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-28 |
Detector | Bruker PHOTON II |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 3 |
Unit cell lengths | 103.452, 103.452, 103.452 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.740 - 1.200 |
R-factor | 0.175 |
Rwork | 0.174 |
R-free | 0.18930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7s3u |
RMSD bond length | 0.010 |
RMSD bond angle | 1.596 |
Data reduction software | SAINT |
Data scaling software | SADABS |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.300 |
High resolution limit [Å] | 1.200 | 1.200 |
Number of reflections | 57433 | 12226 |
<I/σ(I)> | 13.7 | 4.2 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 12 | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG-8000, 2% MPD, 5 mM dTDP, 5 mM acetyl coenzyme A, 100 mM MOPS |