7S32
Crystal structure of hen egg white lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 125 |
| Detector technology | CCD |
| Collection date | 2020-12-12 |
| Detector | OXFORD TITAN CCD |
| Wavelength(s) | 1.54056 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.184, 77.184, 37.104 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.370 - 1.600 |
| R-factor | 0.1908 |
| Rwork | 0.189 |
| R-free | 0.22280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6qwy |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.769 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 13.380 | 13.370 | 1.630 |
| High resolution limit [Å] | 1.600 | 8.760 | 1.600 |
| Rmerge | 0.037 | 0.025 | 0.188 |
| Total number of observations | 443 | 2239 | |
| Number of reflections | 15195 | 84 | 726 |
| <I/σ(I)> | 22.7 | 48.2 | 5.2 |
| Completeness [%] | 99.1 | 71.8 | 98.2 |
| Redundancy | 4.9 | 5.3 | 3.1 |
| CC(1/2) | 0.999 | 0.999 | 0.948 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.6 | 295 | 1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection |
