7S30

Crystal structure of hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	125
Detector technology	CCD
Collection date	2020-12-12
Detector	OXFORD TITAN CCD
Wavelength(s)	1.54056
Spacegroup name	P 43 21 2
Unit cell lengths	78.654, 78.654, 36.949
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.580 - 1.600
R-factor	0.1906
Rwork	0.189
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qwy
RMSD bond length	0.011
RMSD bond angle	1.706
Data reduction software	HKL-2000
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	13.590	13.580	1.630
High resolution limit [Å]	1.600	8.760	1.600
Rmerge	0.040	0.018	0.278
Total number of observations		466	2198
Number of reflections	15709	87	746
<I/σ(I)>	22.8	79.1	3.7
Completeness [%]	99.2	70.7	98.9
Redundancy	4.7	5.4	2.9
CC(1/2)	0.999	1.000	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	295	1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection