7S2W

Crystal structure of hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	125
Detector technology	CCD
Collection date	2020-12-12
Detector	OXFORD TITAN CCD
Wavelength(s)	1.54056
Spacegroup name	P 43 21 2
Unit cell lengths	77.209, 77.209, 37.235
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.380 - 1.600
R-factor	0.2059
Rwork	0.204
R-free	0.25060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 6qwy
RMSD bond length	0.009
RMSD bond angle	1.525
Data reduction software	HKL-2000
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	13.380	13.380	1.630
High resolution limit [Å]	1.600	8.620	1.600
Rmerge	0.124	0.142	0.427
Total number of observations	70799	479	2250
Number of reflections	15187	83	766
<I/σ(I)>	6.3	15.9	1.4
Completeness [%]	99.0	67.9	98.3
Redundancy	4.7	5.8	2.9
CC(1/2)	0.983	0.971	0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	295	1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection