7S2Q

Crystal structure of hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	125
Detector technology	CCD
Collection date	2020-12-12
Detector	OXFORD TITAN CCD
Wavelength(s)	1.54056
Spacegroup name	P 43 21 2
Unit cell lengths	78.299, 78.299, 37.062
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.540 - 1.600
R-factor	0.1888
Rwork	0.187
R-free	0.21440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qwy
RMSD bond length	0.010
RMSD bond angle	1.689
Data reduction software	HKL-2000
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	13.540	13.540	1.630
High resolution limit [Å]	1.600	8.470	1.600
Rmerge	0.060	0.023	0.735
Total number of observations	71019	507	2258
Number of reflections	15699	105	785
<I/σ(I)>	15	59.8	1.5
Completeness [%]	99.7	76.3	98.3
Redundancy	4.5	4.8	2.9
CC(1/2)	0.998	0.997	0.589

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	295	1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection