7S2C
Crystal structure of hen egg white lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | BRUKER IMUS 3.0 MICROFOCUS |
Temperature [K] | 125 |
Detector technology | CMOS |
Collection date | 2020-12-12 |
Detector | BRUKER PHOTON 100 |
Wavelength(s) | 1.54184 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.447, 78.447, 36.951 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.190 - 1.600 |
R-factor | 0.1804 |
Rwork | 0.179 |
R-free | 0.21000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6qwy |
RMSD bond length | 0.012 |
RMSD bond angle | 1.762 |
Data reduction software | XDS |
Data scaling software | SAINT (V8.40A) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 22.190 | 22.190 | 1.654 |
High resolution limit [Å] | 1.597 | 3.438 | 1.597 |
Number of reflections | 30056 | 3115 | 30056 |
<I/σ(I)> | 17.69 | ||
Completeness [%] | 99.9 | ||
Redundancy | 9.85 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 4.6 | 295 | 1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection |