7S29
Crystal structure of hen egg white lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER IMUS 3.0 MICROFOCUS |
| Temperature [K] | 125 |
| Detector technology | CMOS |
| Collection date | 2020-12-12 |
| Detector | BRUKER PHOTON 100 |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.247, 78.247, 36.948 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.420 - 1.600 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.21760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6qwy |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.714 |
| Data reduction software | XDS |
| Data scaling software | SAINT (V8.40A) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.420 | 22.450 | 1.653 |
| High resolution limit [Å] | 1.596 | 3.437 | 1.596 |
| Number of reflections | 15713 | 3115 | 29944 |
| <I/σ(I)> | 10.12 | ||
| Completeness [%] | 99.7 | ||
| Redundancy | 9.88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.6 | 295 | 1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection |
