7S22
Crystal structure of alpha-COP-WD40 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.994, 171.822, 71.424 |
| Unit cell angles | 90.00, 99.57, 90.00 |
Refinement procedure
| Resolution | 44.440 - 1.750 |
| R-factor | 0.175 |
| Rwork | 0.173 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j87 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.948 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.440 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.092 | 0.547 |
| Number of reflections | 85435 | 4461 |
| <I/σ(I)> | 8.3 | 2.2 |
| Completeness [%] | 97.0 | |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295.15 | 20% PEG-3350, 0.25M Sodium citrate tribasic dihydrate |
