7S1B

Crystal structure of Epstein-Barr virus glycoproteins gH/gL/gp42-peptide in complex with human neutralizing antibodies 769C2 and 770F7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	89.326, 107.703, 110.650
Unit cell angles	90.00, 93.11, 90.00

Refinement procedure

Resolution	71.320 - 3.030
R-factor	0.2436
Rwork	0.241
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w0k
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.320	3.100
High resolution limit [Å]	3.000	3.000
Number of reflections	40453	2692
<I/σ(I)>	7.1
Completeness [%]	89.7
Redundancy	6.8
CC(1/2)	0.990	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M HEPES pH7.4, 24% PEG3350, 7.5% Glycerol