7S17

Crystal structure of human G3BP1-NTF2 with three mutations- F15W, F33W, and F124W

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92011
Spacegroup name	P 21 21 21
Unit cell lengths	42.652, 71.669, 87.731
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.910 - 2.360
R-factor	0.2249
Rwork	0.222
R-free	0.27140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fcj
RMSD bond length	0.007
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.910	2.500
High resolution limit [Å]	2.360	2.360
Number of reflections	11543	1793
<I/σ(I)>	7.02
Completeness [%]	99.7
Redundancy	6.4
CC(1/2)	0.991	0.349

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	20% PEG 8000, 100 mM HEPES