7S13

Crystal structure of Fab in complex with mouse CD96 dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-31
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	52.030, 131.250, 186.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.770 - 2.120
R-factor	0.219
Rwork	0.217
R-free	0.26450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4aiz 1nc2 6arq
RMSD bond length	0.009
RMSD bond angle	1.247
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	107.400	2.370
High resolution limit [Å]	2.120	2.120
Rmerge	0.112	0.709
Rpim	0.049	0.364
Number of reflections	49907	2495
<I/σ(I)>	9.8	1.8
Completeness [%]	93.6
Redundancy	6.3
CC(1/2)	0.993	0.676

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Sodium citrate tribasic dihydrate, 20% w/v Polyethylene glycol 3,350