7S11

Crystal structure of Fab in complex with mouse CD96 monomer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-06
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	124.930, 154.890, 306.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.200 - 2.580
R-factor	0.2284
Rwork	0.226
R-free	0.27880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mj8 4amk 6arq
RMSD bond length	0.014
RMSD bond angle	2.115
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	153.000	2.960
High resolution limit [Å]	2.580	2.580
Rmerge	0.195	1.422
Rpim	0.082	0.596
Number of reflections	59695	2986
<I/σ(I)>	6.6	1.6
Completeness [%]	88.5
Redundancy	6.6
CC(1/2)	0.992	0.545

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1 M ammonium sulfate, 0.1 M bis-tris pH 5.5, 1% PEG 3350