7S0P
Crystal structure of Porcine Factor VIII C2 Domain Bound to Phosphatidylserine
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2018-02-05 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 1.1158 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 49.072, 68.323, 105.987 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.531 - 1.300 |
| R-factor | 0.1374 |
| Rwork | 0.137 |
| R-free | 0.15590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mo3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.071 |
| Data reduction software | Adxv |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.531 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.073 | 0.764 |
| Rmeas | 0.078 | 0.892 |
| Rpim | 0.029 | 0.440 |
| Number of reflections | 42676 | 1614 |
| <I/σ(I)> | 18.3 | |
| Completeness [%] | 96.5 | |
| Redundancy | 13.3 | |
| CC(1/2) | 0.998 | 0.832 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2:1 drop ratio 0.1 M CHES (pH 7.4) 0.1M magnesium acetate 10% ethanol (v/v) Crystal soaked with 5 mM OPLS |
