7S0K

HAP2 from Cyanidioschyzon merolae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-19
Detector	MAR CCD 130 mm
Wavelength(s)	1.0331
Spacegroup name	C 2 2 21
Unit cell lengths	102.960, 197.010, 74.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.630 - 2.300
R-factor	0.226
Rwork	0.223
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dbs
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.630	2.360
High resolution limit [Å]	2.300	2.300
Rmerge		0.243
Number of reflections	33534	2329
<I/σ(I)>	7.11	0.45
Completeness [%]	98.8	94.5
Redundancy	3.3	3.2
CC(1/2)	0.993	0.225

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	293.15	PEG3350, isopropanol, glycerol, sodium citrate