7RZX

Crystal Structure of Ferritin grown by microbatch method in presence of agarose and electric field 4.3KV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-02
Detector	ADSC HF-4M
Wavelength(s)	0.979
Spacegroup name	F 4 3 2
Unit cell lengths	181.075, 181.075, 181.075
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.630 - 2.440
R-factor	0.1917
Rwork	0.190
R-free	0.22220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2w0o
RMSD bond length	0.007
RMSD bond angle	0.831
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.630	2.510
High resolution limit [Å]	2.440	2.440
Number of reflections	9949	704
<I/σ(I)>	0.828
Completeness [%]	99.8
Redundancy	20
CC(1/2)	0.998	0.766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		290	CdSO4,NH4SO4,Tris pH7.4