7RZP

Crystal structure of putative NAD(P)H-flavin oxidoreductase from Haemophilus influenzae R2866

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-15
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97919
Spacegroup name	P 31
Unit cell lengths	56.170, 56.170, 122.490
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.640 - 1.950
R-factor	0.1733
Rwork	0.171
R-free	0.21630
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.197
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.980
High resolution limit [Å]	1.950	1.950
Number of reflections	31406	1548
<I/σ(I)>	13.5
Completeness [%]	99.9
Redundancy	5
CC(1/2)	0.978	0.361

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M ammonium acetate; 20% PEG3350