7RZP
Crystal structure of putative NAD(P)H-flavin oxidoreductase from Haemophilus influenzae R2866
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-15 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 31 |
| Unit cell lengths | 56.170, 56.170, 122.490 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.640 - 1.950 |
| R-factor | 0.1733 |
| Rwork | 0.171 |
| R-free | 0.21630 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.197 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 31406 | 1548 |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 99.9 | |
| Redundancy | 5 | |
| CC(1/2) | 0.978 | 0.361 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M ammonium acetate; 20% PEG3350 |
