7RYL
T cell receptor CO3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.93222 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.987, 76.414, 113.148 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.470 - 2.000 |
| R-factor | 0.2011 |
| Rwork | 0.199 |
| R-free | 0.24690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4x6b |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.629 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.470 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rpim | 0.743 | |
| Number of reflections | 30668 | 2241 |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.997 | 0.538 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.15M Ammonium sulfate, 15% PEG 4000, 0.1 MES pH 6 |
