7RXZ
human Hsp90_MC domain structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97922 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 153.830, 87.987, 166.178 |
| Unit cell angles | 90.00, 114.36, 90.00 |
Refinement procedure
| Resolution | 48.090 - 3.152 |
| R-factor | 0.1927 |
| Rwork | 0.191 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q6m |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.405 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.260 |
| High resolution limit [Å] | 3.150 | 6.780 | 3.150 |
| Rmerge | 0.132 | 0.073 | 0.811 |
| Rmeas | 0.158 | 0.088 | 0.973 |
| Rpim | 0.086 | 0.049 | 0.531 |
| Total number of observations | 231203 | ||
| Number of reflections | 70092 | 7186 | 6937 |
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 99.7 | 99.2 | 99.7 |
| Redundancy | 3.3 | 3.4 | 3.2 |
| CC(1/2) | 0.985 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M NaAc PH 6.0, 12 % PEG3350 |
