7RXY
Hen egg-white lysozyme with ionic liquid ethylammonium nitrate 5 mol%
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.531, 62.477, 59.648 |
| Unit cell angles | 90.00, 90.26, 90.00 |
Refinement procedure
| Resolution | 29.841 - 1.600 |
| Rwork | 0.140 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7jmu |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.866 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.140 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 26661 | 1365 |
| <I/σ(I)> | 10.1 | 4.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.5 | |
| CC(1/2) | 0.999 | 0.950 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 293.15 | ionic liquid ethylammonium nitrate 5 mol% (ca. 24wt%) in water |
