7RXE

Crystal structure of junctophilin-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-11-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	P 32 2 1
Unit cell lengths	63.121, 63.121, 149.677
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.850 - 2.350
R-factor	0.2196
Rwork	0.217
R-free	0.26060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rw4
RMSD bond length	0.006
RMSD bond angle	0.840
Data reduction software	xia2
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	54.664	37.419	2.480
High resolution limit [Å]	2.350	7.430	2.350
Rmerge		0.106	1.435
Rmeas	0.314	0.114	1.520
Rpim	0.105	0.041	0.496
Total number of observations	131028	4153	19618
Number of reflections	15049	555	2138
<I/σ(I)>	5.4	11.8	1.5
Completeness [%]	100.0	99.1	100
Redundancy	8.7	7.5	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG4000, sodium citrate, isopropanol