7RX1
Crystal structure of the TIR domain from the grapevine disease resistance protein RUN1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-26 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 152.435, 41.471, 77.232 |
Unit cell angles | 90.00, 118.51, 90.00 |
Refinement procedure
Resolution | 33.930 - 1.890 |
R-factor | 0.202 |
Rwork | 0.201 |
R-free | 0.22630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6o0w |
RMSD bond length | 0.008 |
RMSD bond angle | 0.807 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.930 | 1.959 |
High resolution limit [Å] | 1.890 | 1.892 |
Rmerge | 0.062 | 1.262 |
Rmeas | 0.074 | 0.074 |
Rpim | 0.040 | 0.801 |
Number of reflections | 34128 | 3294 |
<I/σ(I)> | 12.06 | 0.94 |
Completeness [%] | 99.5 | 97.6 |
Redundancy | 3.4 | 3.4 |
CC(1/2) | 0.997 | 0.358 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.2 M Lithium Sulfate, 0.1 M HEPES pH 7, 20% PEG3350 |