7RWY
Crystal structure of a Fe-bound RIDC1 variant in the presence of reductant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.550, 82.680, 74.140 |
| Unit cell angles | 90.00, 95.22, 90.00 |
Refinement procedure
| Resolution | 44.650 - 2.200 |
| R-factor | 0.2052 |
| Rwork | 0.202 |
| R-free | 0.25920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bc5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.159 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.270 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.072 | |
| Rmeas | 0.110 | 0.434 |
| Rpim | 0.054 | 0.216 |
| Number of reflections | 30358 | 4390 |
| <I/σ(I)> | 8 | |
| Completeness [%] | 99.1 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.992 | 0.863 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 40% PPG, 0.1 M Bis-Tris |
