7RWU

Crystal structure of Ni-bound RIDC1 variant in the presence of reductant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-07-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 41 3 2
Unit cell lengths	94.260, 94.260, 94.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.150 - 1.800
R-factor	0.1654
Rwork	0.164
R-free	0.18920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bc5
RMSD bond length	0.010
RMSD bond angle	0.994
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.150	1.800
High resolution limit [Å]	1.800	1.800
Rmerge	0.069	0.191
Rmeas	0.070	0.193
Rpim	0.011	0.032
Number of reflections	25101	1344
<I/σ(I)>	58.1
Completeness [%]	100.0
Redundancy	68
CC(1/2)	1.000	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	295	45% MPD, 0.1 M Tris, 0.2 M MgCl2