7RWS

Structure of SAVED domain of Cap5 from Lactococcus lactis in complex with cGAMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	80
Detector technology	CCD
Collection date	2020-10-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	C 2 2 21
Unit cell lengths	68.696, 126.713, 83.439
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.040 - 1.800
R-factor	0.1681
Rwork	0.167
R-free	0.19090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rwk
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.052
Number of reflections	34016	3297
<I/σ(I)>	16.4
Completeness [%]	99.7
Redundancy	5.7
CC(1/2)	0.990	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	10-12.5% PEG6000, 100 mM Tris-HCl, pH 8.5