7RVJ

Segment from the human prion protein 169-175 YSNQNNF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ELECTRON MICROSCOPE
Source details	OTHER
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-08-25
Detector	TVIPS TEMCAM-F416
Wavelength(s)	0.0251
Spacegroup name	P 1
Unit cell lengths	24.390, 4.950, 20.860
Unit cell angles	86.52, 77.16, 85.68

Refinement procedure

Resolution	11.870 - 1.000
R-factor	0.226
Rwork	0.225
R-free	0.23300
Structure solution method	AB INITIO PHASING
RMSD bond length	0.010
RMSD bond angle	0.910
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	11.870	11.870	1.030
High resolution limit [Å]	1.000	4.500	1.010
Rmerge	0.283	0.178	0.478
Rmeas	0.308	0.189	0.573
Number of reflections	4309	40	204
<I/σ(I)>	4.49	9.17	1.72
Completeness [%]	85.8	78.4	56.5
Redundancy	5.518	7.525	2.309
CC(1/2)	0.957	0.984	0.336

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	298	0.1 M sodium/potassium phosphate, pH 6.2, 10% w/v PEG3000