7RVI

Segment from naked mole rat (elk T174S) prion protein 168-176 QYNNQNSFV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ELECTRON MICROSCOPE
Source details	OTHER
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-06-28
Detector	TVIPS TEMCAM-F416
Wavelength(s)	0.0251
Spacegroup name	C 1 2 1
Unit cell lengths	62.760, 4.850, 21.520
Unit cell angles	90.00, 109.14, 90.00

Refinement procedure

Resolution	10.760 - 1.050
R-factor	0.2271
Rwork	0.222
R-free	0.26480
Structure solution method	AB INITIO PHASING
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	10.760	10.760	1.080
High resolution limit [Å]	1.050	4.700	1.050
Rmerge	0.287	0.152	0.589
Rmeas	0.305	0.161	0.623
Total number of observations	21917
Number of reflections	2612	18	187
<I/σ(I)>	5	7.99	2.47
Completeness [%]	79.9	37.5	83.5
Redundancy	8.391	6.222	8.561
CC(1/2)	0.981	0.991	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.1 M sodium cacodylate, pH 6.5, 5% isopropanol, 0.1 M zinc acetate