7RVH

Q172E mutant of the bank vole prion protein 168-176 QYNNENNFV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ELECTRON MICROSCOPE
Source details	OTHER
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-02-15
Detector	TVIPS TEMCAM-F416
Wavelength(s)	0.0251
Spacegroup name	P 1
Unit cell lengths	4.870, 10.060, 30.660
Unit cell angles	94.85, 90.26, 99.99

Refinement procedure

Resolution	7.980 - 0.900
R-factor	0.222
Rwork	0.221
R-free	0.23500
Structure solution method	AB INITIO PHASING
RMSD bond length	0.009
RMSD bond angle	1.306
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	7.980	7.980	0.930
High resolution limit [Å]	0.900	1.930	0.900
Rmerge	0.197	0.151	0.475
Rmeas	0.214	0.163	0.529
Total number of observations	18502
Number of reflections	3437	350	330
<I/σ(I)>	5.21	8.61	2.56
Completeness [%]	81.0	79	82.3
Redundancy	5.383	5.834	4.858
CC(1/2)	0.988	0.986	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	0.1 M lithium sulfate, 2.5 M sodium chloride, 0.1 M sodium acetate, pH 4.5