7RVA

Updated Crystal Structure of Replication Initiator Protein REPE54.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2020-11-24
Detector	RDI CMOS_8M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	107.578, 80.715, 73.299
Unit cell angles	90.00, 122.63, 90.00

Refinement procedure

Resolution	33.780 - 1.890
R-factor	0.2096
Rwork	0.207
R-free	0.24890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rep
RMSD bond length	0.009
RMSD bond angle	1.107
Data reduction software	XDS (Jan 31, 2020)
Data scaling software	XSCALE (Jan 31, 2020)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.780	1.930
High resolution limit [Å]	1.890	1.890
Rmerge	0.050	0.442
Rmeas	0.068	0.600
Rpim	0.046	0.403
Number of reflections	41783	2702
<I/σ(I)>	10.3	2.6
Completeness [%]	98.8	99.6
Redundancy	3.7	3.6
CC(1/2)	0.998	0.728

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	300 mM MgCl2, 18% PEG 400, and 100 mM Tris HCl