7RUQ

Structure of the human GIGYF1-TNRC6C complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	C 1 2 1
Unit cell lengths	99.918, 32.503, 69.578
Unit cell angles	90.00, 133.84, 90.00

Refinement procedure

Resolution	29.630 - 1.790
R-factor	0.1665
Rwork	0.166
R-free	0.18930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rup
RMSD bond length	0.011
RMSD bond angle	1.238
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.630	1.830
High resolution limit [Å]	1.790	1.790
Number of reflections	15377	861
<I/σ(I)>	11.2
Completeness [%]	99.5	94.3
Redundancy	6.5	5.9
CC(1/2)	0.997	0.747

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	100 mM Hepes 7.0, 1 M sodium malonate