7RUM
Endolysin from Escherichia coli O157:H7 phage FTEbC1, LysT84
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2019-03-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 106.928, 106.928, 369.259 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.040 - 2.990 |
| R-factor | 0.2346 |
| Rwork | 0.233 |
| R-free | 0.27220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nm7 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.519 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19-40) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.040 | 49.040 | 3.170 |
| High resolution limit [Å] | 2.990 | 8.970 | 2.990 |
| Rmerge | 0.521 | 0.092 | 3.403 |
| Rmeas | 0.535 | 0.095 | 3.509 |
| Rpim | 0.124 | 0.024 | 0.841 |
| Total number of observations | 487418 | 18573 | 70198 |
| Number of reflections | 26401 | 1164 | 4115 |
| <I/σ(I)> | 6.6 | 19.7 | 1.1 |
| Completeness [%] | 99.9 | 99.5 | 99.5 |
| Redundancy | 18.5 | 16 | 17.1 |
| CC(1/2) | 0.974 | 0.971 | 0.554 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | Morpheus I condition F3: 0.12 M monosaccharides [0.2 M D-glucose; 0.2 M D-mannose; 0.2 M D-galactose; 0.2 M L-fucose; 0.2 M D-xylose; 0.2 M N-acetyl-D-glucosamine] 0.1 M buffer System 1 [1.0 M imidazole; MES monohydrate (acid) pH 6.5], 30% v/v Precipitant Mix 3 [40% v/v glycerol; 20% w/v PEG 4000 |
