7RTI

X-ray structure of RBPJ-L3MBTL3(dT62)-DNA complex

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	277
Detector technology	PIXEL
Collection date	2019-11-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	67.790, 97.080, 105.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.450 - 2.050
R-factor	0.1898
Rwork	0.188
R-free	0.22280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3iag
RMSD bond length	0.008
RMSD bond angle	0.936
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.080	2.096
High resolution limit [Å]	2.050	2.051
Number of reflections	43800	2556
<I/σ(I)>	10.6
Completeness [%]	98.7
Redundancy	6.3
CC(1/2)	0.997	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	ammonium fluoride PEG 3350