7RTH

Crystal structure of an anti-lysozyme nanobody in complex with an anti-nanobody Fab "NabFab"

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-11
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	225.530, 130.280, 223.980
Unit cell angles	90.00, 107.99, 90.00

Refinement procedure

Resolution	90.907 - 3.190
R-factor	0.2185
Rwork	0.216
R-free	0.26710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jb9 5c8j
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	111.430	3.360
High resolution limit [Å]	3.190	3.190
Rpim	0.170	0.737
Number of reflections	102005	14566
<I/σ(I)>	9.5	1.9
Completeness [%]	99.4
Redundancy	5.7	5.4
CC(1/2)	0.980	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	292	0.02 M Citric acid, 0.08 M BIS-TRIS propane, 16 % (w/v) PEG3350