7RTC

Crystal structure of the ARM domain from Drosophila SARM1 in complex with NaMN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-02
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.95373
Spacegroup name	P 1 21 1
Unit cell lengths	67.122, 102.823, 100.198
Unit cell angles	90.00, 103.84, 90.00

Refinement procedure

Resolution	39.410 - 3.310
R-factor	0.2394
Rwork	0.238
R-free	0.25690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7lcy
RMSD bond length	0.004
RMSD bond angle	0.600
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.410	3.580
High resolution limit [Å]	3.310	3.310
Number of reflections	18295	3462
<I/σ(I)>	6.6
Completeness [%]	92.0
Redundancy	6.7
CC(1/2)	0.990	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	1.7 M sodium malonate pH 5.8