7RT0
1.80 A resolution structure of MAO from P. nicotinovorans in complex with FAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 203.302, 51.740, 45.739 |
| Unit cell angles | 90.00, 92.57, 90.00 |
Refinement procedure
| Resolution | 45.690 - 1.800 |
| R-factor | 0.1701 |
| Rwork | 0.169 |
| R-free | 0.19330 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (dev_4273) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.690 | 45.690 | 1.840 |
| High resolution limit [Å] | 1.800 | 9.000 | 1.800 |
| Rmerge | 0.145 | 0.070 | 1.011 |
| Total number of observations | 303398 | 2484 | 18664 |
| Number of reflections | 44295 | 385 | 2637 |
| <I/σ(I)> | 8.6 | 21.3 | 2 |
| Completeness [%] | 100.0 | 99 | 100 |
| Redundancy | 6.8 | 6.5 | 7.1 |
| CC(1/2) | 0.995 | 0.995 | 0.757 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 298 | 20% (w/v) PEG 6000, 0.1 M Bicine |
