Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2019-12-01 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.049, 120.775, 195.558 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.890 - 2.790 |
| R-factor | 0.2369 |
| Rwork | 0.236 |
| R-free | 0.25880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tgr |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | 2.910 |
| High resolution limit [Å] | 2.790 | 2.800 |
| Rmerge | 0.176 | |
| Number of reflections | 43101 | 4401 |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.25 | |
| CC(1/2) | 0.999 | 0.457 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 23% PEG 1500 (w/v), 0.1 M CaCl2, 0.1 M imidazole pH 6.5 |
