7RSW
Crystal structure of group B human rotavirus VP8*
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97741 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.277, 116.257, 31.312 |
| Unit cell angles | 90.00, 103.54, 90.00 |
Refinement procedure
| Resolution | 30.410 - 1.320 |
| R-factor | 0.1598 |
| Rwork | 0.157 |
| R-free | 0.20830 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.835 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.410 | 1.340 |
| High resolution limit [Å] | 1.320 | 1.320 |
| Rmerge | 0.040 | 0.262 |
| Number of reflections | 41757 | 1675 |
| <I/σ(I)> | 19.03 | 2.31 |
| Completeness [%] | 82.7 | |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M ammonium sulfate, 30% PEG 2000 MME, 0.1 M sodium acetate, pH 4.6 |
