7RSK
The crystal structure from microfluidic crystals of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.228, 231.147, 67.558 |
| Unit cell angles | 90.00, 109.02, 90.00 |
Refinement procedure
| Resolution | 39.860 - 2.400 |
| R-factor | 0.1849 |
| Rwork | 0.183 |
| R-free | 0.22040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBID 6B6L |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.482 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.165 | 0.858 |
| Number of reflections | 57327 | 2796 |
| <I/σ(I)> | 8.9 | 1.75 |
| Completeness [%] | 87.3 | 84.5 |
| Redundancy | 3.9 | 3 |
| CC(1/2) | 0.447 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROFLUIDIC | 8 | 289 | 0.2 M potassium chloride and 20% (w/v) PEG3350 |
