7RRK

Crystal structure of fast switching M159E mutant of fluorescent protein Dronpa (Dronpa2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	79.825, 85.732, 143.050
Unit cell angles	90.00, 95.05, 90.00

Refinement procedure

Resolution	37.730 - 1.929
R-factor	0.2435
Rwork	0.242
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uts
RMSD bond length	0.005
RMSD bond angle	1.060
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.13rc2_2986)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.284	1.998
High resolution limit [Å]	1.929	1.929
Rmerge	0.135	1.700
Rmeas	0.140	1.770
Rpim	0.039	0.500
Number of reflections	139109	13363
<I/σ(I)>	10.8	1.2
Completeness [%]	95.9	92.7
Redundancy	12.5	11.5
CC(1/2)	0.998	0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	292	0.1 M Tris-HCl pH 7.4, 0.1 M MgCl2, 18% PEG 3350