7RRJ
Crystal structure of fast switching M159Q mutant of fluorescent protein Dronpa (Dronpa2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.745, 85.789, 144.045 |
| Unit cell angles | 90.00, 95.14, 90.00 |
Refinement procedure
| Resolution | 36.890 - 2.200 |
| R-factor | 0.243 |
| Rwork | 0.242 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uts |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.062 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13RC2_2986) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.742 | 2.280 |
| High resolution limit [Å] | 2.198 | 2.200 |
| Rmerge | 0.268 | 1.860 |
| Number of reflections | 96708 | 6925 |
| <I/σ(I)> | 10.5 | 1.2 |
| Completeness [%] | 93.3 | 68.4 |
| Redundancy | 12.4 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 292 | 0.1 M Tris-HCl pH 7.4, 0.1 M MgCl2, 18% PEG 3350 |
