7RQF
Crystal Structure of LbcA (lipoprotein binding partner of CtpA) of Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-08-09 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 120.699, 120.699, 221.943 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.390 - 3.500 |
| R-factor | 0.247 |
| Rwork | 0.245 |
| R-free | 0.28060 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.608 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19_4092)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.570 | 3.690 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.183 | |
| Number of reflections | 24325 | 3487 |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 22.1 | |
| CC(1/2) | 0.999 | 0.566 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.1 M sodium acetate, pH 4.6, and 1.9 M ammonium dihydrogen phosphate at a concentration of 45 mg/mL |
