7RQB

Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site aminoacyl-tRNA analog ACC-PMN, and P-site MAI-tripeptidyl-tRNA analog ACCA-IAM at 2.45A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-12
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	209.610, 448.450, 618.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	187.310 - 2.450
R-factor	0.2072
Rwork	0.205
R-free	0.24420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
RMSD bond length	0.004
RMSD bond angle	0.871
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	187.310	187.310	2.510
High resolution limit [Å]	2.450	10.960	2.450
Rmerge	0.207	0.096	2.080
Rmeas	0.224	0.104	2.241
Total number of observations	14684473
Number of reflections	2100555	24476	154835
<I/σ(I)>	6.75	17.78	1
Completeness [%]	99.9	98.9	100
Redundancy	6.991	6.977	7.196
CC(1/2)	0.989	0.988	0.218

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME