7RPU

Crystal Structure of Protective Human Antibody 3A6 Fab Against Ebola Virus with GP Stalk/MPER Epitope Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-04-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	52.293, 66.427, 67.981
Unit cell angles	90.00, 104.24, 90.00

Refinement procedure

Resolution	46.780 - 1.270
R-factor	0.1422
Rwork	0.141
R-free	0.15980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SWISS model
RMSD bond length	0.008
RMSD bond angle	1.028
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.890	1.320
High resolution limit [Å]	1.270	1.270
Number of reflections	111264	3378
<I/σ(I)>	11	2.29
Completeness [%]	93.9
Redundancy	2
CC(1/2)	0.999	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	30% PEG 3000, 0.1 M Tris, pH 7.0, and 0.2 M sodium chloride