7RPT

Crystal Structure of Protective Human Antibody 3A6 Fab Against Ebola Virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-08-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033167
Spacegroup name	P 1
Unit cell lengths	53.659, 65.674, 125.556
Unit cell angles	98.74, 91.37, 96.01

Refinement procedure

Resolution	43.510 - 2.500
R-factor	0.2008
Rwork	0.198
R-free	0.25410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SWISS Model
RMSD bond length	0.013
RMSD bond angle	1.670
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	123.998	2.733
High resolution limit [Å]	2.500	2.500
Number of reflections	38276	1915
<I/σ(I)>	2.7
Completeness [%]	78.1
Redundancy	1.7
CC(1/2)	0.987	0.525

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	28% PEG400, 0.1M HEPES:NaOH, pH7.5 and 0.2M Calcium Chloride