7RPR
Borrelia miyamotoi FbpA complement inhibitory domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2018-03-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.975 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.428, 40.320, 74.086 |
| Unit cell angles | 90.00, 105.41, 90.00 |
Refinement procedure
| Resolution | 35.710 - 1.900 |
| R-factor | 0.2079 |
| Rwork | 0.206 |
| R-free | 0.23250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6n1l |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.250 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.18.2_3874) |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.166 | 0.654 |
| Rpim | 0.068 | 0.276 |
| Number of reflections | 25861 | 2566 |
| <I/σ(I)> | 13.2 | 2.13 |
| Completeness [%] | 97.3 | |
| Redundancy | 5.8 | |
| CC(1/2) | 0.988 | 0.834 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 290.15 | 12.8mg/mL, 0.1M tris (8.5), 2M ammonium sulfate |
