7ROA
Crystal structure of EntV136 from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 62.394, 32.064, 49.992 |
| Unit cell angles | 90.00, 90.86, 90.00 |
Refinement procedure
| Resolution | 31.190 - 1.820 |
| R-factor | 0.1768 |
| Rwork | 0.175 |
| R-free | 0.21710 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.858 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmeas | 0.138 | 0.290 |
| Number of reflections | 8895 | 451 |
| <I/σ(I)> | 14.2 | 6.9 |
| Completeness [%] | 98.6 | 99.1 |
| Redundancy | 6.2 | |
| CC(1/2) | 0.913 | 0.618 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.1 M Tris pH 8 and 28% (w/v) PEG4K, cryoprotectant paratone-N oil |
