7RMN

Crystal structure of triosephosphate isomerase from Verrucomicrobium spinosum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2021-02-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95372
Spacegroup name	P 1 21 1
Unit cell lengths	48.990, 64.118, 94.215
Unit cell angles	90.00, 100.79, 90.00

Refinement procedure

Resolution	46.280 - 1.210
R-factor	0.1644
Rwork	0.164
R-free	0.17880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b9b
RMSD bond length	0.005
RMSD bond angle	0.863
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.120	1.290
High resolution limit [Å]	1.210	1.210
Number of reflections	170138	26066
<I/σ(I)>	12.15
Completeness [%]	98.6
Redundancy	6.8
CC(1/2)	0.998	0.928

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2 M Ammonium citrate dibasic, 20% w/v Polyethylene glycol 3,350, pH 5.1